LCMS-TQMS based Quantification Assay

QMAC’s instrumentation includes hybrid triple quadrupole-linear ion trap mass spectrometers, such as the SCIEX 6500 QTRAP and SCIEX 7500 QTRAP, which are ideal for high-throughput metabolite quantification and identification. Below is a list of panels we have developed so far. Link to the full list can be downloaded from here.

1. Amino Acids and related compounds
2. Eicosanoids
3. NAD+/NADH
4. S-Geranylgeranyl-L-glutathione and metabolic derivaties
5. Methotrexate and metabolic derivatives

GCMS-Orbitrap based Quantification Assay

GCMS is a new addition to the core, and we're actively developing methods for short-chain fatty acids. If you have questions about method details or instrument availability, please feel free to contact us.

For qualitative exploration and high-resolution experiments, the SCIEX TripleTOF 6600+ platform offers accurate mass and tandem mass spectrometry capabilities, enabling comprehensive detection and identification of metabolites across diverse biological systems.

Metabolomics

We offer global, unbiased profiling of small molecules - ranging from polar to mid–non-polar compounds - to capture the biochemical state of cells, tissues, or biofluids. This enables researchers to discover novel biomarkers, characterize metabolic pathways, and compare metabolic signatures across experimental groups.

Lipidomics

Specialized workflows enable detailed analysis of lipid classes and species with high mass accuracy and sensitivity. Our lipidomics platform supports studies on membrane biology, energy metabolism, and disease-related lipid dysregulation.

Isotope Tracing Analysis

Stable isotope-labeled substrates can be tracked to monitor dynamic metabolic fluxes. This approach reveals pathway utilization, turnover rates, and substrate channeling, providing deep insights into cellular metabolism under physiological and pathological conditions.

Advanced Statistical Analysis

Comprehensive data processing and multivariate statistical tools are applied to extract biologically meaningful patterns. We offer support for interpretation, visualization, and integration of metabolomics datasets with other omics platforms. For further information, please consult Dr. Lu Yang.

We are dedicated to creating custom panels tailored to the unique needs of our investigators. Our team works closely with researchers to design assays that address specific biological questions, ensuring sensitivity, reproducibility, and scalability.

QMAC In-house MS Library

QMAC has developed a comprehensive compound library sourced from a wide range of materials, enabling us to deliver high-precision and robust data analysis for both targeted and untargeted studies. A full list of compounds in our library can be downloaded here.

Semi-Automated MS Library Build Workflow

QMAC established semi-automated workflow to streamline library build and assay setup, reducing variability and increasing throughput. This system allows rapid optimization and standardization of assay conditions, ensuring reliable and reproducible results.

A Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI) spectrometer (Biotyper sirius CA System) enables detection of metabolites in non-liquid matrices, including feces or solid tumors. 

QMAC offers access to a variety of small instruments available in our lab. Please see below for descriptions and availability. If you're interested in using any of the equipment, feel free to reach out to Jacob Brockert.

Labconco Refrigerated CentriVap Vacuum Concentrator

This concentrator is designed to rapidly concentrate multiple small, heat-sensitive samples. It is equipped with a -105°C cold trap to enhance efficiency.

Labconco FreeZone Triad Benchtop Freeze Dryer

This unit supports both stoppering tray drying and sample freeze drying, with four sample valves. It allows for the simultaneous processing of both freeze-drying types.

Eppendorf Centrifuge 5910 Ri

This unit is a refrigerated, high-capacity, multipurpose centrifuge equipped with rotors suitable for a wide range of applications.

Equipment Availability Calendar

View is available here

This training program is designed for advanced users who wish to gain independent access to our instruments with maximum scheduling flexibility and minimal supervision. Superusers are trusted and proficient operators who understand not only the technical use of the equipment but also the lab’s safety and data quality standards.

If you have any questions, feel free to contact Ju Eun Jeon, or find more details here. 

Note: The pricing listed below reflects our current rates, but it is subject to change. Pricing is adjusted semi-annually and cannot be guaranteed. For projects scheduled to begin more than three months after a quote is generated, we recommend adding a 10–15% pricing buffer to account for potential increases in service costs. Final pricing will be determined at the time the work is completed by the facility.

* Includes 26% Facilities and Administration Rate

** Includes 10% Surplus Revenue and 26% Facilities and Administration Rate

QMAC was founded by the following PIs: Jason Cyster, Andrei Goga, Susan Lynch, and Peter Turnbaugh. Darren Dumlao is the Assistant Director of QMAC.

QMAC is made possible by support from the Benioff Center for Microbiome Medicine, ImmunoX, and the Program for Breakthrough Biomedical Research (PBBR).