QMAC
Quantitative Metabolite Analysis Center
Metabolomics CoLab Plug-in

Overview

As a UCSF Collaborative Laboratory (CoLab) Plug-in, the Quantitative Metabolite Analysis Center (QMAC) is a full-service metabolomics hub at the Parnassus campus, equipped with state-of-the-art mass spectrometers for targeted and untargeted metabolomics. Our platforms enable the development of frameworks for predicting disease, distinguishing disease phenotypes, deciphering biochemical mechanisms of health and disease, and the discovery of new drugs and drug targets.

TripleTOF equipment

Instrumentation includes two hybrid triple quadrupole – linear ion trap mass spectrometers, a SCIEX 6500 QTRAP and a SCIEX 7500 QTRAP, ideally suited for high throughput quantification and metabolite identification. A hybrid triple-quadrupole time-of-flight platform, SCIEX TripleTOF 6600+ is available for high-resolution accurate mass and tandem mass spectrometry, optimized for qualitative exploration and high-resolution experimentation. A Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI) spectrometer (Biotyper sirius CA System) detects metabolites in non-liquid matrices e.g. feces or solid tumors. A new gas chromatography mass spectrometer (GC-Orbitrap) has been purchased and will be operational in 2024, expanding our capacity to detect small, non-polar metabolites more suited for ionization in the gas phase (e.g. short chain fatty acids). 

The QMAC is equipped for sample preparation and chemical extractions with access to chemical storage facilities, chemical fume hoods, biosafety cabinets, cell culture hoods and incubators. Staff-operated services available include study design, sample preparation, sample extractions, LCMS operation and method development, and data analysis. We also offer consultations and training opportunities in metabolomics workflow design and data analysis. UCSF researchers that frequently use the QMAC have the ability to become designated trained users and access the instruments independently. 

Custom Panels

We are continuously developing custom panels to serve our investigators. Below is a list of panels we've developed to date. 

Branched chain amino acids
Phenylalanine metabolites
Cardenolides
​Midazolam
Methotrexate and metabolic derivatives
Sulfasalazine and metabolic derivatives
5-Fluorouracil and metabolic derivatives
Lipids
- 12,13-Dihome
- S-Geranylgeranyl-L-glutathione
- Sphingolipids
- Ceramides

Tryptophan metabolism
- Kynurenine pathway
- Decarboxylation pathway
- IAA pathway
- Hydroxylation pathway
- Gut bacterial metabolites (ILA, IPA, IALD)
Radiation response (brain tumor therapy)
- 2-hydroxyglutamic acid
- Riboflavin
- Gamma amino butyric acid
- Succinic Acid
Microbial Derived Metabolites

Pricing Structure

Below is a summary of our approved recharge rates, effective July 1, 2023.

Note: The pricing listed below is our current pricing. However, pricing is adjusted semi-annually and is not guaranteed. For projects that will be processed >3 months from the time of quote generation, we suggest that you add a 10-15% pricing buffer to account for increasing service costs. Actual pricing will be determined at the time the work will be completed by the facility.

Services:  Units:  Internal Rates:  External Rates:  External For Profit Rates:
Metabolite extraction  per sample  $55.49 $69.92  $76.91
Untargeted  per sample  $109.29 $137.71 $151.48
Targeted  per sample  $57.01 $71.83 $79.02
Lipidomics Targeted  per sample  $67.65  $85.23 $93.76
QMAC Staff Hourly Rate, Targeted  per hour  $179.07  $225.63 $248.19
QMAC Staff Hourly Rate, Lipidomics Targeted  per hour  $206.67 $206.40 $286.44
Trained User Hourly Rate, Targeted  per hour  $137.91 $173.76 NA
Trained User Hourly Rate, Lipidomics Targeted  per hour  $191.08 $240.77 NA
Run Only Hourly Rate, Targeted per hour  $86.86 $109.44 NA
Run Only Hourly Rate, Lipidomics Targeted per hour  $127.63 $160.81 NA
Analysis (hours)  per hour  $141.59  $178.40 $196.25
Tech prep time (hours)  per hour  $58.67 $73.92 $81.31
Consultations/Experimental design/Training (hours)  per hour  $172.16 $216.92 $238.61
Special Consumables each $105.02 $132.33 $145.56

 

Next Steps

To discuss new or ongoing projects, submit a Request Project Support form on iLab and schedule a 30-minute consultation with Dr. Darren Dumlao, Assistant Director. Please note that consultations are charged via an hourly rate.

Ready to get started? Submit a request on iLab.

 

Meet our team

Darren Dumlao, PhD
Assistant Director

Alex Campbell
Staff Research Associate

Jacob Brockert
Staff Research Associate