Overview
The Quantitative Metabolite Analysis Center (QMAC), a UCSF Collaborative Laboratory (CoLab) Plug-in, is a comprehensive metabolomics hub located at the Parnassus campus. It is equipped with advanced mass spectrometry tools for both targeted and untargeted metabolomics. These platforms support research aimed at predicting disease, understanding disease phenotypes, exploring biochemical mechanisms in health and disease, and discovering new drugs and drug targets.
QMAC provides facilities for sample preparation and chemical extractions, including chemical storage, fume hoods, and biosafety cabinets. Services offered by the center include study design, sample preparation, chemical extractions, LCMS operation, method development, and data analysis. Researchers can also benefit from consultations and training in designing metabolomics workflows and analyzing data. Frequent users of QMAC may become trained designated users, gaining independent access to the instruments.
Services
Targeted Panel Assays
LCMS-TQMS based Quantification Assay
QMAC’s instrumentation includes hybrid triple quadrupole-linear ion trap mass spectrometers, such as the SCIEX 6500 QTRAP and SCIEX 7500 QTRAP, which are ideal for high-throughput metabolite quantification and identification. Below is a list of panels we have developed so far. Link to the full list can be downloaded from the link below.
- Amino Acids and related compounds
- 5-Fluorouracil (5-FU) metabolism related compounds
- Eicosanoids
- NAD+/NADH
- S-Geranylgeranyl-L-glutathione and metabolic derivaties
- Methotrexate and metabolic derivatives
GCMS-Orbitrap based Quantification Assay
GCMS is a new addition to the core, and we're actively developing methods for short-chain fatty acids. If you have questions about method details or instrument availability, please feel free to contact us.
Untargeted Metabolite Profiling
For qualitative exploration and high-resolution experiments, the SCIEX TripleTOF 6600+ platform offers accurate mass and tandem mass spectrometry capabilities, enabling comprehensive detection and identification of metabolites across diverse biological systems.
Metabolomics
We offer global, unbiased profiling of small molecules - ranging from polar to mid–non-polar compounds - to capture the biochemical state of cells, tissues, or biofluids. This enables researchers to discover novel biomarkers, characterize metabolic pathways, and compare metabolic signatures across experimental groups.
Lipidomics
Specialized workflows enable detailed analysis of lipid classes and species with high mass accuracy and sensitivity. Our lipidomics platform supports studies on membrane biology, energy metabolism, and disease-related lipid dysregulation.
Isotope Tracing Analysis
Stable isotope-labeled substrates can be tracked to monitor dynamic metabolic fluxes. This approach reveals pathway utilization, turnover rates, and substrate channeling, providing deep insights into cellular metabolism under physiological and pathological conditions.
Advanced Statistical Analysis
Comprehensive data processing and multivariate statistical tools are applied to extract biologically meaningful patterns. We offer support for interpretation, visualization, and integration of metabolomics datasets with other omics platforms. For further information, please consult Dr. Lu Yang.
High-Throughput Panel Assay Development
We are dedicated to creating custom panels tailored to the unique needs of our investigators. Our team works closely with researchers to design assays that address specific biological questions, ensuring sensitivity, reproducibility, and scalability.
QMAC In-house MS Library
QMAC has developed a comprehensive compound library sourced from a wide range of materials, enabling us to deliver high-precision and robust data analysis for both targeted and untargeted studies. A full list of compounds in our library is in preparation and will be available soon.
Semi-Automated MS Library Build Workflow
QMAC established semi-automated workflow to streamline library build and assay setup, reducing variability and increasing throughput. This system allows rapid optimization and standardization of assay conditions, ensuring reliable and reproducible results.
MALDI Strain Identification
A Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI) spectrometer (Biotyper sirius CA System) enables detection of metabolites in non-liquid matrices, including feces or solid tumors.
Small Equipment Access
QMAC offers access to a variety of small instruments available in our lab. Please see below for descriptions and availability. If you're interested in using any of the equipment, feel free to reach out to Jacob Brockert.
Labconco Refrigerated CentriVap Vacuum Concentrator
This concentrator is designed to rapidly concentrate multiple small, heat-sensitive samples. It is equipped with a -105°C cold trap to enhance efficiency.
Labconco FreeZone Triad Benchtop Freeze Dryer
This unit supports both stoppering tray drying and sample freeze drying, with four sample valves. It allows for the simultaneous processing of both freeze-drying types.
Eppendorf Centrifuge 5910 Ri
This unit is a refrigerated, high-capacity, multipurpose centrifuge equipped with rotors suitable for a wide range of applications.
Equipment Availability Calendar
View is available here
Pricing Structure
Meet our team
Darren Dumlao, PhD
Assistant Director
Jacob Brockert
Assistant Specialist
Ju Eun Jeon, PhD
Manager
Lu Yang, PhD
Computational Biologist
Next Steps
To discuss new or ongoing projects, submit a Request Project Support form on iLab and schedule a 30-minute consultation with Dr. Darren Dumlao, Assistant Director (login is required to access the schedule). Please note that consultations are charged via an hourly rate.
Ready to get started? Submit a request on iLab.
Publications
Leadership and Funding
Useful Links